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Gastrointestinal NeuroendocrineTumors

Chapter 89 | Oncology and Hematology · Part 4 – Oncology: Solid Tumors · Part 4 – Oncology: Solid Tumors · Chapter 89


Key Clinical Points

  1. GI NETs are classified as extrapancreatic (carcinoid) or pancreatic based on origin.
  2. Grading is determined by mitotic count and Ki-67 index (grades 1–3).
  3. Functional NETs secrete hormones (e.g., insulinoma, gastrinoma), while nonfunctional ones are asymptomatic until metastasis.
  4. MEN1 syndrome is the most common inherited condition linked to NETs.
  5. Diagnosis involves chromogranin A, 5-HIAA, and somatostatin receptor imaging.
  6. Surgical resection is primary for localized disease; somatostatin analogues manage functional tumors.
  7. Appendiceal NETs <2 cm have low metastasis risk and may require only appendectomy.
  8. Well-differentiated NETs (e.g., small intestine) show improved survival compared to historical data.
  9. Somatostatin receptor scintigraphy shows >90% of NETs express receptors; 68Ga dotatate PET has high sensitivity for primary and metastatic disease.
  10. Telotristat ethyl is specifically used to treat carcinoid syndrome diarrhea.

1. DEFINITION & OVERVIEW

Gastrointestinal neuroendocrine tumors (NETs) are classified by site and differentiation:

Extrapancreatic NETs: Historically called carcinoid tumors; arise in stomach, small intestine, appendix, and rectum. • Pancreatic NETs: Subcategorized as functional (hormone-secreting) or nonfunctional (asymptomatic until metastasis).

Grading is determined by mitotic count and Ki-67 index:

Grade 1: <2 mitoses/10 HPF, Ki-67 <3% • Grade 2: 2–20 mitoses/10 HPF, Ki-67 3–20% • Grade 3: >20 mitoses/10 HPF, Ki-67 >20%

Definition (Harrison's 22e): Gastroenteropancreatic neuroendocrine tumors comprise the majority of neuroendocrine tumors, and recent analyses suggest the incidence in this subgroup has risen proportionately and continues to rise.

1.1 Classification by Site

Extrapancreatic: Stomach, small intestine, appendix, rectum. • Pancreatic: Functional (insulinoma, gastrinoma) vs nonfunctional. • Rare Secretions: Somatostatin, VIP, ACTH, or PTH.

Refer to Table 89-1 for detailed histologic classification including differentiation and grade.

1.2 Classification by Differentiation and Grade

Well-differentiated NETs: Monotonous sheets of small round cells with uniform nuclei. • Poorly differentiated (carcinoid): Pleomorphic cells, high mitotic count. • Immunohistochemistry: Positive for chromogranin A/synaptophysin. • Ultrastructure: Electron-dense neurosecretory granules.


2. EPIDEMIOLOGY

Incidence and trends:

SEER data (1973–2012): 6.4-fold increase in overall NET incidence. • Estimated prevalence: >170,000 patients with diagnosed NETs. • Gastroenteropancreatic NETs account for the majority of all NETs.

Risk factors:

Environmental factors: No clear link to diet, tobacco, or alcohol. • Inherited syndromes: 5–10% of NETs occur in inherited conditions (MEN1, VHL, NF1).

2.1 Risk Factors

No clear association with lifestyle factors. • Inherited syndromes: ◦ MEN1 (40–50% of cases) ◦ VHL (<5%) ◦ NF1 (<5%)

Age-adjusted incidence in US increased from 1975–2015. • Gastroenteropancreatic NETs show the steepest increase (3-fold rise).


3. ETIOLOGY & PATHOPHYSIOLOGY

Genetic alterations:

Pancreatic NETs: 44% have MEN1 mutations; 43% have DAXX/ATRX mutations. • mTOR pathway mutations in 15% of pancreatic NETs. • Extrapancreatic NETs: Rare recurrent mutations (CDKN1B in 8% of small intestinal NETs).

Inherited syndromes:

MEN1: Mutation in menin gene (chromosome 11q13). Patients develop pancreatic NETs, hyperparathyroidism, and pituitary adenomas. • VHL: Chromosome 3p25 mutation; associated with renal cancer, hemangioblastomas, and rare pancreatic NETs.

3.1 Inherited Genetic Syndromes

MEN1 syndrome: Mutation in menin gene (chromosome 11q13). Patients develop pancreatic NETs, hyperparathyroidism, and pituitary adenomas. • VHL syndrome: Chromosome 3p25 mutation; associated with renal cancer, hemangioblastomas, and rare pancreatic NETs. • NF1: Neurofibromin mutations; increased risk of both pancreatic and extrapancreatic NETs.

3.2 Sporadic Mutations

Small intestinal NETs: Loss of chromosome 18, epigenetic changes (clinical significance uncertain). • Familial small intestinal NETs: Multiple synchronous tumors; no identified mutation in most cases.


4. CLINICAL FEATURES

Functional vs nonfunctional tumors:

Functional (20% of pancreatic): Hormone-related symptoms (hypoglycemia, diarrhea, flushing). • Nonfunctional (80% of pancreatic): Asymptomatic until metastasis; diagnosed incidentally or with abdominal pain/weight loss.

Carcinoid syndrome:

Symptoms: Flushing (face/neck erythema), diarrhea, right-sided valvular heart disease. • Triggers: Stress, alcohol, cheese. • Consequences: Diarrhea → dehydration, hypokalemia, achlorhydria.

4.1 Functional Pancreatic NETs

Insulinoma: Hypoglycemia with confusion, coma; weight gain. • Glucagonoma: Necrolytic migratory erythema (NME), glucose intolerance, weight loss. • Gastrinoma: Zollinger-Ellison syndrome (peptic ulcers, diarrhea). • VIPoma: Verner-Morrison syndrome (watery diarrhea, hypokalemia, achlorhydria). • ACTHoma: Cushing's syndrome (hyperglycemia, weight gain, hypokalemia).

4.2 Nonfunctional Pancreatic NETs

• Asymptomatic until metastasis. • Often discovered on imaging for unrelated conditions or with abdominal pain/weight loss.

4.3 Gastric NETs

Type 1: Chronic atrophic gastritis, pernicious anemia, elevated gastrin. • Type 2: Associated with gastrinoma; multifocal tumors. • Type 3: Solitary tumors, normal gastrin levels, aggressive behavior.

4.4 Extrapancreatic NETs

Small bowel NETs: Terminal ileum origin; difficult to diagnose early; associated with mesenteric fibrosis. • Appendiceal NETs: Incidental finding in appendectomy specimens (<2 cm diameter, low metastasis risk). • Rectal NETs: Common in Asia (up to 90% of NETs); small size (<1 cm), rarely metastatic.


5. DIFFERENTIAL DIAGNOSIS

Insulinoma mimics:

Severe liver diseaseAlcoholismSurreptitious insulin use

Gastrinoma mimics:

Achlorhydria (chronic atrophic gastritis)PPI use

Carcinoid syndrome mimics:

Systemic mastocytosisChronic laxative/diuretic abuse


6. INVESTIGATIONS & DIAGNOSIS

Diagnostic approach:

  1. Laboratory Markers:Chromogranin A: Elevated in metastatic NETs (Note: Not specific; elevated in PPI use, renal failure). • 5-HIAA: Urinary serotonin metabolite for carcinoid syndrome. • Plasma markers: ◦ Glucagon (>1000 pg/mL) → confirms glucagonoma. ◦ Somatostatin → indicates somatostatinoma.

  2. Imaging Modalities:CT/MRI: Standard for anatomical assessment. • Somatostatin receptor scintigraphy: >90% of NETs express receptors. • 68Ga dotatate PET: High sensitivity for primary and metastatic disease. • FDG PET: ◦ Low sensitivity in well-differentiated NETs. ◦ Positive in high-grade tumors.

6.1 Laboratory Markers

Chromogranin A: Elevated in metastatic NETs (not specific). • 5-HIAA: Urinary serotonin metabolite for carcinoid syndrome. • Plasma glucagon: >1000 pg/mL confirms glucagonoma. • Plasma somatostatin: Elevated in somatostatinoma.

6.2 Imaging Modalities

CT/MRI: Standard for anatomical assessment. • Somatostatin receptor scintigraphy: >90% of NETs express receptors. • 68Ga dotatate PET: High sensitivity for primary and metastatic disease. • FDG PET: Low sensitivity in well-differentiated NETs.

6.3 Diagnostic Criteria for Specific Syndromes

Insulinoma: Fasting hyperinsulinemia with elevated proinsulin/C-peptide. • Gastrinoma: Fasting hypergastrinemia. • Glucagonoma: Plasma glucagon >1000 pg/mL. • VIPoma: Elevated plasma VIP. • Somatostatinoma: Elevated plasma somatostatin. • Carcinoid syndrome: Elevated 5-HIAA.


7. MANAGEMENT & TREATMENT

  1. Localized Disease: • Primary treatment: Surgical resection. • Appendiceal NETs (<2 cm): Appendectomy alone sufficient (low metastasis risk). • Rectal NETs (>2 cm or lymph node involvement): Right colectomy.

  2. Functional Tumors: • Somatostatin analogues (octreotide, lanreotide) → control hormone secretion. • Telotristat ethyl → treatment for carcinoid syndrome diarrhea.

  3. Insulinoma Specific Management: • Diazoxide: Inhibits insulin release; side effects include sodium retention. • Everolimus: Improves glycemic control.

  4. Systemic & Advanced Therapy: • Chemotherapy: For high-grade NETs (e.g., streptozocin, doxorubicin). • Targeted therapy: Everolimus for advanced NETs. • Radioembolization: For liver metastases.

7.1 Pharmacologic Management

Somatostatin analogues: ◦ Octreotide 25–50 μg SC TID. ◦ Lanreotide 30 mg IM every 4 weeks. • Telotristat ethyl: 125 mg PO TID for carcinoid syndrome diarrhea. • Everolimus: 10 mg PO daily for insulinoma.

7.2 Surgical Management

Complete resection for localized disease (pancreatic NETs, appendiceal NETs <2 cm). • Right colectomy for rectal NETs with >2 cm diameter or lymph node involvement.


8. PROGNOSIS & COMPLICATIONS

Survival data:

Small intestine NETs: 5-year survival ~50–70%. • Appendiceal NETs <2 cm: >90% 5-year survival.

Complications:

Carcinoid heart disease: Right-sided valvular fibrosis (tricuspid/pulmonary). • Mesenteric fibrosis: Leads to intestinal obstruction or ischemia if vasculature is involved. • Metastatic disease: Prognosis worsens with increasing grade.

Clinical Findings

Mesenteric Fibrosis: Often presents as a 'spoke and wheel' calcified mass on CT (Figure 4B).


9. SPECIAL CONSIDERATIONS

MEN1 syndrome:

40–50% of patients develop pancreatic NETs. • Surveillance: Annual endoscopic ultrasound, MRI, and biochemical testing.

Other syndromes:

VHL: Pancreatic NETs occur in <5% of cases. • NF1: Increased risk of both pancreatic and extrapancreatic NETs.


10. KEY PEARLS & CLINICAL TRAPS

Diagnostic pitfalls:

Chromogranin A elevation eq specific (can be caused by PPI use or renal failure). • 5-HIAA elevation is not diagnostic of carcinoid syndrome on its own.

Treatment traps:

Somatostatin analogues → worsen hypoglycemia in insulinoma patients. • Appendiceal NETs <2 cm rarely metastasize; avoid over-treatment/unnecessary surgery.

Trial Data Summary (Tables 89-3 & 89-5):

Lanreotide: 65% vs 33% at 2 years (p <0.001). • Cabozantinib: Significant improvement in progression-free survival for both epNET and pNET. • Sunitinib: 11.4 vs 5.5 months (p <0.001). • Temozolomide/capecitabine: 22.7 vs 14.4 months (p = 0.021). • Everolimus + octreotide: 16.4 vs 11.3 months. • Surufatinib: 9.2 vs 3.8 months (p <0.0001). • Pazopanib: 11.6 vs 8.5 months (p <0.005). • 177Lutetium dotatate: 65.2 vs 10.8% at 20 months (p <0.001).


Reference Tables

TABLE 88-4 Combination Chemotherapy Regimens That Have an Impact on Survival in Stage IV Disease

Harrison's 22e, p.676

STUDY DESIGN (AUTHOR/REF) NO. OF
PATIENTS
MEDIAN OVERALL
SURVIVAL (MONTHS)
Gemcitabine + erlotinib vs gemcitabine
(Moore et al: J Clin Oncol 26:1960, 2007)
569 6.24 vs 5.91 (HR 0.82;
95% CI 0.69–0.99;
p = .038)
342
Nab-paclitaxel + gemcitabine vs
gemcitabine (Von Hoff et al: N Eng J Med
369:1691, 2013)
861 8.5 vs 6.7 (HR 0.72;
95% CI 0.62–0.83;
p <.001a
417
NALIRIFOX (nanoliposomal irinotecan,
5-fluorouracil, folinic acid, oxaliplatin) vs
nab-paclitaxel and gemcitabine (Wainberg
et al: Lancet 402:1272, 2023)
770 11.1 vs 9.2 (HR 0.83;
95% CI 0.70–0.99;
p = .036)
89 Gastrointestinal
Neuroendocrine Tumors
Matthew H. Kulke

TABLE 89-1 Histologic Classification of Neuroendocrine Tumors CLASSIFICATION Neuroendocrine tumor Neuroendocrine tumor…

Harrison's 22e, p.677

CLASSIFICATION DIFFERENTIATION GRADE MITOTIC COUNT KI-67
Neuroendocrine tumor Well differentiated Low grade (grade 1) <2 per 10 HPF <3%
Well differentiated Intermediate grade (grade 2) 2–20 per 10 HPF
Neuroendocrine tumor Well differentiated High grade (grade 3) >20 per 10 HPF >20%
Poorly differentiated High grade (grade 3) >20 per 10 HPF

TABLE 89-2 Clinical Presentation and Management of Secretory Syndromes Associated with Neuroendocrine Tumors

Harrison's 22e, p.678

CLINICAL SYMPTOMS
AND MANIFESTATIONS
TREATMENT OPTIONS
TO CONTROL
SECRETORY SYMPTOMS
Pancreatic Neuroendocrine Tumors
Gastrinoma (generally
located in “gastrinoma
triangle”)
Zollinger-Ellison
syndrome:
gastroesophageal reflux,
peptic ulcer disease,
diarrhea
Proton pump inhibitors,
somatostatin analogues
Hypoglycemia leading
to confusion, lethargy,
coma; weight gain
Glucagonoma Skin rash (necrolytic
migratory erythema),
glucose intolerance,
weight loss
Somatostatin analogues
Verner-Morrison
syndrome: watery
diarrhea, hypokalemia,
achlorhydria
ACTHoma Cushing’s syndrome:
hyperglycemia, weight
gain, hypokalemia
Ketoconazole,
metyrapone, consider
adrenalectomy
Extrapancreatic Gastrointestinal Neuroendocrine Tumors

TABLE 89-3 Selected Randomized Trials of Therapeutic Agents for the Treatment of Advanced Neuroendocrine Tumors (NETs)

Harrison's 22e, p.684

TUMOR TYPE NUMBER OF
PATIENTS
PROGRESSION-FREE
SURVIVAL
Pancreatic and Extrapancreatic NETs
Lanreotide vs placebo
(CLARINET)
204 65% vs 33% at 2 years
(p <.001)
226 (limited to
histologic grade 2
and 3 tumors)
Cabozantinib vs placebo
(CABINET)
298 (203
extrapancreatic
NETs [epNET] and
95 pancreatic NETs
[pNET])
8.5 vs 4 months (epNET);
13.8 vs 4.5 months (pNET)
(p <.0001 for both cohorts)
Pancreatic NET
410
Sunitinib vs placebo 171 11.4 vs 5.5 months (p <.001)
264
Temozolomide/
capecitabine vs
temozolomide
144 22.7 vs 14.4 months (p = .021)
Extrapancreatic NET
85
Everolimus + octreotide vs
octreotide (RADIANT 2)
429 16.4 vs 11.3 months
302
Surufatinib vs placebo 198 9.2 vs 3.8 months (p <.0001)
171
177-Lutetium dotatate vs
octreotide (NETTER 1)
230 65.2 vs 10.8% at 20 months
(p <.001)