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Non-Hodgkin’s Lymphoma

Chapter 113 | Part 4: Oncology and Hematology

KEY CLINICAL POINTS

  • NHL is a heterogeneous group of malignancies of mature B, T, and NK cells, distinct from Hodgkin’s lymphoma.
  • 5-year survival rate is 74% for Caucasians, higher than for African Americans.
  • WHO-HAEM5 classification includes B-cell and T-cell subtypes with specific genetic and morphologic features.
  • Key risk factors include immunosuppression, HIV, EBV, HTLV-1, and chronic infections like H. pylori.
  • Treatment varies by subtype: R-CHOP for DLBCL, BR for FL, and CAR-T for relapsed/refractory cases.

1. DEFINITION & OVERVIEW

Non-Hodgkin’s lymphoma (NHL) encompasses cancers of mature B, T, and NK cells. Distinguished from Hodgkin’s lymphoma (HL) by the absence of Reed-Sternberg cells and differing biologic/clinical features. NHL is classified into B-cell and T-cell subtypes based on cell origin. WHO-HAEM5 classification includes 12 categories (Table 113-1).

WHO-HAEM5 Classification of Lymphoid Malignancies

B CELL T CELL
Lymphoplasmacytic lymphoma (Waldenström’s macroglobulinemia) T-cell granular lymphocytic leukemia
Hairy cell leukemia (HTLV-1+) Adult T-cell leukemia/lymphoma (HTLV-1+)
Splenic marginal zone B-cell lymphoma Extranodal NK/T-cell lymphoma, nasal type
Extranodal marginal zone B-cell lymphoma of MALT type Enteropathy-associated T-cell lymphoma
Nodal marginal zone B-cell lymphoma Hepatosplenic T-cell lymphoma
Follicular lymphoma Subcutaneous panniculitis-like T-cell lymphoma
Mantle cell lymphoma Mycosis fungoides
Diffuse large B-cell lymphoma (including subtypes) Sezary syndrome
High-grade B-cell lymphoma with MYC and BCL2 rearrangements Peripheral T-cell lymphoma NOS
High-grade B-cell lymphoma NOS Angioimmunoblastic T-cell lymphoma
High-grade B-cell lymphoma with 11q aberrations Anaplastic large-cell lymphoma, ALK+
Burkitt’s lymphoma/Burkitt’s cell leukemia Anaplastic large-cell lymphoma, ALK–
B CELL T CELL
Primary mediastinal large B-cell lymphoma Plasmablastic lymphoma
Mediastinal grey zone lymphoma Primary effusion lymphoma
Primary large B-cell lymphoma of immune-privileged sites HHV8+ DLBCL NOS
Intravascular large B-cell lymphoma Intravascular large B-cell lymphoma

1.1 WHO-HAEM5 Classification

B-cell and T-cell subtypes include diffuse large B-cell lymphoma (DLBCL), follicular lymphoma (FL), mantle cell lymphoma (MCL), and T-cell lymphomas like anaplastic large-cell lymphoma (ALCL).

2. EPIDEMIOLOGY

NHL is the 7th most common cancer in the US (80,550 new cases in 2023). Incidence is 10x higher in immunosuppressed patients (e.g., post-transplant). Male-to-female ratio ~1:1.5. Age >40, with peak incidence in 60–70 years. 20–40% increase in incidence over 20–40 years. Higher in Caucasians vs. African Americans.

Diseases/Exposures Associated with NHL Risk

Inherited Immunodeficiency Acquired Immunodeficiency Chemical/Drug Exposures
Klinefelter’s syndrome HIV Phenytoin
Chédiak-Higashi syndrome Autoimmune diseases Dioxin, phenoxy herbicides
Ataxia-telangiectasia Iatrogenic immunosuppression Radiation
Wiskott-Aldrich syndrome Acquired hypogammaglobulinemia Prior chemotherapy/radiation
Common variable immunodeficiency Anti-TNF drugs N/A

2.1 Risk Factors

Immunosuppression (HIV, organ transplant, autoimmune diseases), EBV, HTLV-1, H. pylori, hepatitis C, and genetic predispositions (e.g., ataxia-telangiectasia).

3. ETIOLOGY & PATHOPHYSIOLOGY

NHL arises from genetic mutations in B/T/NK cells, often involving translocations (e.g., t(11;14) in MCL), mutations in MYC, BCL2, and other oncogenes. EBV and HTLV-1 are linked to specific subtypes (e.g., BL, ATL). Chronic antigenic stimulation (e.g., H. pylori) drives MALT lymphoma.

Genetic Features of B- and T-Cell Lymphomas

GENETIC FEATURE GENES LYMPHOMA
t(8;14) MYC/IgH Burkitt’s lymphoma
t(2;8) MYC/Igk T-cell ALL
t(8;22) MYC/Igl T-cell ALL
t(11;14) BCL1 (CCND1)/IgH Mantle cell lymphoma
t(14;18) BCL2/IgH Follicular lymphoma, DLBCL
t(3;14) BCL6/IgH DLBCL
GENETIC FEATURE GENES LYMPHOMA
Trisomy 3 Unknown Splenic marginal zone lymphoma
7q21 deletion CDK6 Splenic marginal zone lymphoma
inv(14) TCL1 Peripheral T-cell lymphoma, NOS
t(2;5) NPM1/ALK Anaplastic large-cell lymphoma
t(1;2) TPM3/ALK Anap, large-cell lymphoma
t(2;17) CTLC/ALK Anap, large-cell lymphoma
inv(2) ATIC/ALK Anap, large-cell lymphoma
Trisomy 5 Unknown Angioimmunoblastic T-cell lymphoma
Isochromosome 7q Unknown Hepatosplenic T-cell lymphoma

3.1 Genetic Abnormalities

Common translocations include t(11;14) (MCL), t(14;18) (FL), and t(8;14) (BL). MYC, BCL2, and BCL6 mutations drive oncogenesis.

4. CLINICAL FEATURES

Symptoms include B symptoms (fever, night sweats, weight loss), lymphadenopathy, and extranodal involvement. Indolent NHLs (e.g., FL) present with painless lymphadenopathy, while aggressive subtypes (e.g., BL) present with rapid growth and systemic symptoms.

4.1 Subtype-Specific Features

Burkitt’s lymphoma: rapid growth, jaw/abdominal mass. FL: indolent, stage I/II. MCL: splenomegaly, lymphadenopathy. ALCL: CD30+ cells, ALK positivity.

5. DIFFERENTIAL DIAGNOSIS

Differentiate from reactive follicular hyperplasia, autoimmune conditions (e.g., Sjögren’s), and infections (e.g., EBV-related lymphomas). Consider other B-cell disorders like CLL and MZL.

6. INVESTIGATIONS & DIAGNOSIS

CBC, LDH, β -microglobulin, and imaging (CT/PET). Flow cytometry and immunohistochemistry for B/T-cell markers. Ann Arbor staging (Table 113-6) and IPI/FLIPI for prognosis.

Ann Arbor Staging for Lymphoma

STAGE DESCRIPTION
I Single lymph node region (I) or single extranodal site (IE)
II Two or more lymph node regions or limited extralymphatic involvement (IIE)
III Lymph node regions on both sides of the diaphragm (III), including spleen (IIIS) or extralymphatic sites (IIIE)
IV Diffuse or disseminated foci with lymphatic involvement

6.1 Diagnostic Criteria

WHO classification, genetic testing (FISH for translocations), and staging with CT/PET.

7. MANAGEMENT & TREATMENT

R-CHOP for DLBCL, BR for FL, and CAR-T for relapsed/refractory cases. Targeted therapies (e.g., ibrutinib for MZL) and stem cell transplants for high-risk patients. PET/CT for response assessment.

7.1 Subtype-Specific Therapies

DLBCL: R-CHOP; FL: BR; MCL: R-CHOP + autologous transplant; ALCL: CHOP/CHOEP; PTCL NOS: CHOP/CHOEP.

8. PROGNOSIS & COMPLICATIONS

IPI and FLIPI predict outcomes. Complications include CNS involvement, treatment resistance, and secondary malignancies. Prognosis varies by subtype (e.g., 82% 8-year OS for ALK+ ALCL vs. 49% for ALK–).

8.1 Prognostic Indices

IPI: age, LDH, performance status, stage, extranodal involvement. FLIPI: age, stage, LDH, number of nodal sites, B symptoms.

9. SPECIAL CONSIDERATIONS

Pregnancy: avoid chemotherapy in first trimester. Pediatric: aggressive treatment for high-risk subtypes. Elderly: consider less toxic regimens. HIV-positive patients require antiretroviral therapy alongside NHL treatment.

10. KEY POINTS & CLINICAL PEARLS

  1. NHL is heterogeneous, with distinct B/T-cell subtypes. 2. EBV, HTLV-1, and chronic infections are key etiologic factors. 3. R-CHOP is standard for DLBCL; BR for FL. 4. PET/CT guides treatment decisions. 5. CAR-T and targeted therapies improve outcomes in relapsed/refractory cases.